There is a trend in microscopes towards larger field-if-view and larger image circles to be able to receive more information with the recording of a single image. For that reason, camera manufacturers followed in designing and creating new scientific cameras with larger image sensors and diagonals as well. But, not only the diagonal but even more the pixel size has to be selected properly according to the applied microscope magnification. The relationship will be discussed within this webinar.Further, it is known that all microscope systems have an optical transfer function or modulation transfer function, and so do cameras. But what is the impact on modern microscope methods like single molecule localization microscopy and structured illumination microscopy? This question has been experimentally investigated and results will be discussed.Finally, can the extension to the short wave infrared (SWIR) range of the spectrum be used with microscopes to have a better or deeper look into thick samples to see the invisible? Some applications based on new SWIR image sensors and light microscopes will be shown.